sequence shrna Search Results


shrna sequence  (OriGene)
90
OriGene shrna sequence
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrna Sequence, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/10__1158_slash_0008___5472__can___13___1027-66-9-14?v=OriGene
Average 90 stars, based on 1 article reviews
shrna sequence - by Bioz Stars, 2026-08
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22-nt shrna sequences  (Rosetta Inpharmatics)
90
Rosetta Inpharmatics 22-nt shrna sequences
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
22 Nt Shrna Sequences, supplied by Rosetta Inpharmatics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pmc03263926-108-1-7?v=Rosetta+Inpharmatics
Average 90 stars, based on 1 article reviews
22-nt shrna sequences - by Bioz Stars, 2026-08
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shrna sequences trcn0000231750 and trcn0000231753  (Microsynth ag)
90
Microsynth ag shrna sequences trcn0000231750 and trcn0000231753
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrna Sequences Trcn0000231750 And Trcn0000231753, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/bio_rxiv__694380-50-5-28?v=Microsynth+ag
Average 90 stars, based on 1 article reviews
shrna sequences trcn0000231750 and trcn0000231753 - by Bioz Stars, 2026-08
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cd90-overexpression lentiviral plasmids  (VectorBuilder GmbH)
90
VectorBuilder GmbH cd90-overexpression lentiviral plasmids
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Cd90 Overexpression Lentiviral Plasmids, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pmc10822841-237-0-17?v=VectorBuilder+GmbH
Average 90 stars, based on 1 article reviews
cd90-overexpression lentiviral plasmids - by Bioz Stars, 2026-08
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shrna sequences  (GenScript corporation)
90
GenScript corporation shrna sequences
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrna Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pmc06714815-309-38-102?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
shrna sequences - by Bioz Stars, 2026-08
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foxc1 small interfering (si)rna1  (Shanghai GenePharma)
90
Shanghai GenePharma foxc1 small interfering (si)rna1
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Foxc1 Small Interfering (Si)rna1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pm30431099-36-3-40?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
foxc1 small interfering (si)rna1 - by Bioz Stars, 2026-08
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shrna sequences targeting prr34-as1 (sh-prr34as1  (Shanghai GenePharma)
90
Shanghai GenePharma shrna sequences targeting prr34-as1 (sh-prr34as1
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrna Sequences Targeting Prr34 As1 (Sh Prr34as1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/10__2147_slash_cmar__s263619-87-8-23?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
shrna sequences targeting prr34-as1 (sh-prr34as1 - by Bioz Stars, 2026-08
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shorthairpin rna (shrna) sequences targeting krt17  (Shanghai GenePharma)
90
Shanghai GenePharma shorthairpin rna (shrna) sequences targeting krt17
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shorthairpin Rna (Shrna) Sequences Targeting Krt17, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/ppr0559103-60-0-9?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
shorthairpin rna (shrna) sequences targeting krt17 - by Bioz Stars, 2026-08
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shrna sequences  (Broad Institute Inc)
90
Broad Institute Inc shrna sequences
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrna Sequences, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pmc10072906-753-1-23?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
shrna sequences - by Bioz Stars, 2026-08
90/100 stars
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shrna targeting sequences  (Broad Institute Inc)
90
Broad Institute Inc shrna targeting sequences
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrna Targeting Sequences, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pm32719458-353-28-21?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
shrna targeting sequences - by Bioz Stars, 2026-08
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shrna sequence sh-clasrp  (Ribobio co)
90
Ribobio co shrna sequence sh-clasrp
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrna Sequence Sh Clasrp, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pmc10474993-48-1-12?v=Ribobio+co
Average 90 stars, based on 1 article reviews
shrna sequence sh-clasrp - by Bioz Stars, 2026-08
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shrnas targeting different sites within the coding sequences of becn1 and lc3b  (Cyagen Biosciences)
90
Cyagen Biosciences shrnas targeting different sites within the coding sequences of becn1 and lc3b
Figure 3. Inhibitory effect <t>of</t> <t>RHBDF1</t> gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 <t>shRNA</t> (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.
Shrnas Targeting Different Sites Within The Coding Sequences Of Becn1 And Lc3b, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+shrna/pm24262968-216-13-25?v=Cyagen+Biosciences
Average 90 stars, based on 1 article reviews
shrnas targeting different sites within the coding sequences of becn1 and lc3b - by Bioz Stars, 2026-08
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Image Search Results


Figure 3. Inhibitory effect of RHBDF1 gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 shRNA (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.

Journal: Cancer Research

Article Title: Human Rhomboid Family-1 Suppresses Oxygen-Independent Degradation of Hypoxia-Inducible Factor-1α in Breast Cancer

doi: 10.1158/0008-5472.can-13-1027

Figure Lengend Snippet: Figure 3. Inhibitory effect of RHBDF1 gene silencing in MCF7 cells on HIF1a stability and VEGF secretion. A, effect of RHBDF1 shRNA (shRHB) or scrambled shRNA (shScr) on HIF1a degradation in MCF7 cells in hypoxia. B, effect of shRHB or shScr on HIF1a protein stability in MCF7 cells during reoxygenation. C, nuclear translocation of HIF1a in shSrc- or shRHB-treated MCF7 cells; a-tubulin and Myc are cytoplasmic and nuclear protein controls, respectively. D, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MCF7 cells under normoxic or hypoxic conditions as a function of time (hours). E, changes of VEGF concentrations in the condition media of shRHB (white bars)- or shScr (black bars)-treated MDA-MB-231 cells under normoxic or hypoxic conditions as a function of time (hours). Each experiment was repeated two times. , P < 0.05, Student t test.

Article Snippet: Four independent shRNA 29-mers against RHBDF1 and a scrambled shRNA sequence were purchased from OriGene Technologies (cat# TF302009).

Techniques: shRNA, Translocation Assay

Figure 4. Involvement of RHBDF1, RACK1, and HSP90 in oxygen-independent HIF1a degradation. A, effect of RHBDF1 gene silencing in MCF7 cells on the stability of HA-tagged P402A/P564A mutant HIF1a (HA-HIF1a) in hypoxia. B, effect of RHBDF1 overexpression in T47D cells on the stability of the mutant HIF1a in hypoxia. C, effect of RACK1 gene silencing on HIF1a stability in MCF7 cells in hypoxia. D, effect of FLAG-RHBDF1 overexpression in T47D cells on the stability of the mutant HIF1a in hypoxia. E, effect of 17-AAG treatment (0.5 mmol/L, 24 hours) of RHBDF1 gene-silenced MCF7 cells on HIF1a stability in hypoxia. F, effect of 17-AAG treatment (0.5 mmol/L, 24 hours) of RHBDF1-overexpressing T47D cells on HIF1a in hypoxia. G, co-IP of RHBDF1 and RACK1 from MCF7 cells transfected with FLAG-RHBDF1. H, co-IP of RHBDF1 and HIF1a from MCF7 cells cotransfected with HA-tagged wild-type HIF1a. I, co-IP of RHBDF1 with HIF1a and RACK1 from MCF7 cells in the absence or presence of MG132 (10 mmol/L, 4 hours) in normoxia or hypoxia. J, co-IP of HIF1a with HSP90 and of RHBDF1 with RACK1 from MCF7 cells cotransfected with FLAG-RHBDF1 and the mutant HIF1a upon treatment with either control siScr, RACK1 siRNA (siRACK1), or 17-AAG (0.5 mmol/L). Each experiment was repeated two times and representative Western blotting analysis or co-IP results from one experiment are shown.

Journal: Cancer Research

Article Title: Human Rhomboid Family-1 Suppresses Oxygen-Independent Degradation of Hypoxia-Inducible Factor-1α in Breast Cancer

doi: 10.1158/0008-5472.can-13-1027

Figure Lengend Snippet: Figure 4. Involvement of RHBDF1, RACK1, and HSP90 in oxygen-independent HIF1a degradation. A, effect of RHBDF1 gene silencing in MCF7 cells on the stability of HA-tagged P402A/P564A mutant HIF1a (HA-HIF1a) in hypoxia. B, effect of RHBDF1 overexpression in T47D cells on the stability of the mutant HIF1a in hypoxia. C, effect of RACK1 gene silencing on HIF1a stability in MCF7 cells in hypoxia. D, effect of FLAG-RHBDF1 overexpression in T47D cells on the stability of the mutant HIF1a in hypoxia. E, effect of 17-AAG treatment (0.5 mmol/L, 24 hours) of RHBDF1 gene-silenced MCF7 cells on HIF1a stability in hypoxia. F, effect of 17-AAG treatment (0.5 mmol/L, 24 hours) of RHBDF1-overexpressing T47D cells on HIF1a in hypoxia. G, co-IP of RHBDF1 and RACK1 from MCF7 cells transfected with FLAG-RHBDF1. H, co-IP of RHBDF1 and HIF1a from MCF7 cells cotransfected with HA-tagged wild-type HIF1a. I, co-IP of RHBDF1 with HIF1a and RACK1 from MCF7 cells in the absence or presence of MG132 (10 mmol/L, 4 hours) in normoxia or hypoxia. J, co-IP of HIF1a with HSP90 and of RHBDF1 with RACK1 from MCF7 cells cotransfected with FLAG-RHBDF1 and the mutant HIF1a upon treatment with either control siScr, RACK1 siRNA (siRACK1), or 17-AAG (0.5 mmol/L). Each experiment was repeated two times and representative Western blotting analysis or co-IP results from one experiment are shown.

Article Snippet: Four independent shRNA 29-mers against RHBDF1 and a scrambled shRNA sequence were purchased from OriGene Technologies (cat# TF302009).

Techniques: Mutagenesis, Over Expression, Co-Immunoprecipitation Assay, Transfection, Control, Western Blot